Ichigo Mashimaro follows the life of Nobue Itoh, her younger sister Chika, and her friends. The basic premise of the show can be summed up in "cute girls do cute things in cute ways", be it trying to quit smoking, going outside to play, celebrating a holiday, or doing school work.
Seasons play an important role throughout Strawberry Marshmallow as the characters are involved in many normal seasonal activities. The series is speckled with many small, music-related allusions, such as Ana's dog Frusciante being named after John Frusciante of the Red Hot Chili Peppers, while episode eight of the anime sees two goldfish called Richard and James. The title itself was inspired from "Mashimaro", a single by Japanese rock artist Tamio Okuda. The series' focus on young girls, as well as the manga author's history in publications such as Weekly Dearest My Brother, has caused some to classify Strawberry Marshmallow in the lolicon genre.